Aspire, which tastes like cranberry, is said to burn off 209 calories within three hours of drinking it.
Holland & Barrett is selling a 250ml can, which contains just 12.5 calories, for £1.59.
The drink was tested on just 20 people in a £13,500 research project that took over three months.
Scientists at Leeds Metropolitan University found that caffeine and green tea mixed with amino acid and ginger produced a ‘thermogenic effect’.
This means that the body generates more heat to digest food and temporarily stimulates metabolism.
Dr John O’Hara, who led the study, said: ‘The finished case study data from 20 participants suggests that Aspire increases energy expenditure on average of 1.16 calories per minute. Over a three hour period Aspire expends an average of 209 calories.’
A spokesman for Fahrenheit 60, which launched the drink, described it as the UK’s first ‘fully-researched and proven calorie-burning soft drink for the mass market’.
‘Thermogenic products have been used by athletes for many years,’ he added.
‘Yet what we’ve managed to create with Aspire is to bring those calorie burning properties to many more people in a unique and convenient drink with proven functional benefits.
‘It’s an exciting place to be, and could spark more companies to innovate and offer customers new food and drink products which are suited for their lifestyles.’
A spokeswoman said: ‘Functional food is growing fast as customers look for new products which are targeted to their individual lifestyles.
‘Aspire offers proven benefits and could prompt more brands to research new ranges which combine active ingredients for extra functionality.’
In 2006, Coca-Cola and nestle developed a green-tea drink that claimed to burn calories.
Researchers have found that green tea stimulates the brown calorie-burning adipose tissue.
Coca-Cola claimed that drinking three cans of Enviga could help burn 60 to 100 calories. The drink was never launched in the UK.